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src shrna r lentiviral particles  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology src shrna r lentiviral particles
    Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with <t>shRNA</t> targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).
    Src Shrna R Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+shrna+r+lentiviral+particles/SRC-1+shRNA+(r)+Lentiviral+Particles/pm22890232-40-0-14
    Average 90 stars, based on 2 article reviews
    src shrna r lentiviral particles - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Periodic mechanical stress activates integrinβ1-dependent Src-dependent PLCγ1-independent Rac1 mitogenic signal in rat chondrocytes through ERK1/2."

    Article Title: Periodic mechanical stress activates integrinβ1-dependent Src-dependent PLCγ1-independent Rac1 mitogenic signal in rat chondrocytes through ERK1/2.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    doi: 10.1159/000341461

    Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with shRNA targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).
    Figure Legend Snippet: Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with shRNA targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).

    Techniques Used: Expressing, Phospho-proteomics, Activation Assay, Transfection, shRNA, Lysis, Western Blot, Sequencing, Control, Cell Culture, In Vitro, Activity Assay

    Related Articles

    shRNA:

    Article Title: Periodic mechanical stress activates integrinβ1-dependent Src-dependent PLCγ1-independent Rac1 mitogenic signal in rat chondrocytes through ERK1/2.
    Article Snippet: RNAiso Plus, PrimeScript RT Reagent Kit, and SYBR Premix Ex Taq II were purchased from TaKaRa (Japan). .. Src shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles and Polybrene were supplied by Santa Cruz (USA). .. A cell incubator (Hereus BB 5060), air-tight cell culture device and reciprocating pressure pump, barrier type pressure transducer, and inversion microscope equipped with camera system were bought from Hereus (Germany), Taixing Experimental Instrument Factory (China), Tianjin Plastics Research Institute (China), and Olympus (Japan), respectively.

    Article Title: Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1.
    Article Snippet: RNAiso Plus, PrimeScript RT reagent kit, and SYBR Premix Ex Taq II were purchased from TaKaRa (Japan). .. Src shRNA (r) Lentiviral Particles, ERK1 shRNA (r) Lentiviral Particles, ERK2 shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles, and Polybrene were supplied by Santa Cruz. .. A cell incubator (Hereus BB 5060), an air-tight cell culture device and reciprocating pressure pump, a barrier type pressure transducer and inversion microscope equipped with a camera system were purchased from Hereus (Germany), Taixing Experimental Instrument Factory (China), Tianjin Plastics Research Institute (China), and Olympus (Japan), respectively.

    Control:

    Article Title: Periodic mechanical stress activates integrinβ1-dependent Src-dependent PLCγ1-independent Rac1 mitogenic signal in rat chondrocytes through ERK1/2.
    Article Snippet: RNAiso Plus, PrimeScript RT Reagent Kit, and SYBR Premix Ex Taq II were purchased from TaKaRa (Japan). .. Src shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles and Polybrene were supplied by Santa Cruz (USA). .. A cell incubator (Hereus BB 5060), air-tight cell culture device and reciprocating pressure pump, barrier type pressure transducer, and inversion microscope equipped with camera system were bought from Hereus (Germany), Taixing Experimental Instrument Factory (China), Tianjin Plastics Research Institute (China), and Olympus (Japan), respectively.

    Article Title: Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1.
    Article Snippet: RNAiso Plus, PrimeScript RT reagent kit, and SYBR Premix Ex Taq II were purchased from TaKaRa (Japan). .. Src shRNA (r) Lentiviral Particles, ERK1 shRNA (r) Lentiviral Particles, ERK2 shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles, and Polybrene were supplied by Santa Cruz. .. A cell incubator (Hereus BB 5060), an air-tight cell culture device and reciprocating pressure pump, a barrier type pressure transducer and inversion microscope equipped with a camera system were purchased from Hereus (Germany), Taixing Experimental Instrument Factory (China), Tianjin Plastics Research Institute (China), and Olympus (Japan), respectively.



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    src shrna r lentiviral particles  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology src shrna r lentiviral particles
    Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with <t>shRNA</t> targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).
    Src Shrna R Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+shrna+r+lentiviral+particles/SRC-1+shRNA+(r)+Lentiviral+Particles/pm22890232-40-0-14
    Average 90 stars, based on 1 article reviews
    src shrna r lentiviral particles - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with shRNA targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Periodic mechanical stress activates integrinβ1-dependent Src-dependent PLCγ1-independent Rac1 mitogenic signal in rat chondrocytes through ERK1/2.

    doi: 10.1159/000341461

    Figure Lengend Snippet: Fig. 6. Effects of Src on the expression, phosphorylation and activation of Rac1 under conditions of periodic mechanical stress. Chondrocytes were transfected with shRNA targeted to Src or with nontargeting NT sequences prior to lysis and Western blotting for Src protein. The Src shRNA sequence achieved about 50% reduction in Src protein level. After pretreatment with DMSO or Src inhibitor PP2, or Control shRNA or Src shRNA, rat chondrocytes were cultured in vitro for 1 h under static conditions or with periodic mechanical stress. The expression and phosphorylation levels of Rac1 were detected by western blotting, and Rac1 activation was quantiϐied by Rac1 GTPase activity assay. The total quantity of Rac1 served as the control. Results are represented in the histogram (n=5, *, p<0.05 for each). The images above are representative results of western blotting and Rac1 GTPase activity assay. The phosphorylation levels of Rac1-Ser71 and Rac1 activation levels in the PP2 and Src shRNA pretreatment groups were signiϐicantly diminished relative to those of the control groups in response to periodic mechanical stress (n=5, p<0.05 for each, Student unpaired t-test).

    Article Snippet: Src shRNA (r) Lentiviral Particles, Control shRNA Lentiviral Particles and Polybrene were supplied by Santa Cruz (USA).

    Techniques: Expressing, Phospho-proteomics, Activation Assay, Transfection, shRNA, Lysis, Western Blot, Sequencing, Control, Cell Culture, In Vitro, Activity Assay